Retinal Degeneration - Methods and Protocols

This volume provides key updates on several methods from the first edition as well as including new novel techniques to address the most recent technological developments and their applications in retinal research Chapters guide readers through gene identification approaches, detailed protocols to generate functional retinal pigment epithelium cells, mouse retina and other animal models, fundus imaging and angiography, and cell-based treatment approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Retinal Degeneration: Methods and Protocols, Second Edition aims to ensure successful scientific work in the further study of this vital field.

Inhaltsverzeichnis:

Identification and Analysis of Genes Associated with Inherited Retinal Diseases.- eQTL Analysis in Retinal Research.- Testing for known Retinal Degeneration Mutants in Mouse Strains.- CRISPR/Cas9 Gene Editing In Vitro and in Retinal Cells In Vivo.- Monitoring Surface Reactions by Combined Western Blot-ELISA Analysis.- Generation of Functional Retinal Pigment Epithelium from Human Induced Pluripotent Stem Cells.- Advanced Analysis of Photoreceptor Outer Segment Phagocytosis by RPE Cells in Culture.- Porcine RPE/Choroidal Explant Cultures.- The Mouse Retinal Organoid Trisection Recipe: Efficient Generation of 3D Retinal Tissue from Mouse Embryonic Stem Cells.- Cell Death Analysis in Retinal Cultures.- Fate Mapping In Vivo to Distinguish Bona Fide Microglia Versus Recruited Monocyte-derived Macrophages in Retinal Disease.- Light Damage Models of Retinal Degeneration.- Induction and Readout of Oxygen-Iinduced Retinopathy.- Generation and Analysis of Xenopus laevis Models of Retinal De generation using CRISPR/Cas9.- Gene Knockdown in Zebrafish (Danio rerio) as a Tool to Model Photoreceptor Diseases.- Drosophila melanogaster - A Valuable Genetic Model Organism to Elucidate the Biology of Retinitis Pigmentosa.- Retinal Fundus Imaging in Mouse Models of Retinal Diseases.- Phenotyping of Mouse Models with OCT.- Cell-specific Markers for the Identification of Retinal Cells and Subcellular Organelles by Immunofluorescence Microscopy.- Immuno-TEM/STEM in Retinal Research.- Noninvasive Two-photon Microscopy Imaging of Mouse Retina and Retinal Pigment Epithelium.- Cell-based Therapy for Retinal Disease: The New Frontier.- In Vitro Evaluation of AAV Vectors for Retinal Gene Therapy.- Nanoparticles Targeting Retinal and Choroidal Capillaries In Vivo.- Optimized Subretinal Injection Technique for Gene Therapy Approaches.